Our laboratory consists of a 960 square-foot wet bench laboratory fully
equipped for recombinant DNA subcloning and mutagenesis; protein
expression, refolding and purification; steady-state enzymology and
cell culture. We also maintain a number of Linux workstations for NMR
spectral processing, analysis and protein computational biology. We are
currently building a Linux Beowulf cluster for more
computationally-extensive calculations.
As part of the Magnetic Resonance Research Center (MRRC) at the Yale Medical Center, we
maintain and have primary access to a 600 MHz Varian Inova NMR
spectrometer equipped with four identical radiofrequency (RF) channels,
triple-axis (XYZ) pulsed field gradients, and a triple-resonance (HCN)
room temperature probe.
We also share access to a 500 MHz Bruker Avance
NMR spectrometer equipped with three identical radiofrequency (RF)
channels, triple-axis (XYZ)
pulsed field gradients, and an automation unit consisting of a robotic
sample changer and a triple-resonance (HCN) room temperature
probe with a robotic tuning interface.
A variety of addtional NMR spectrometers are housed in the Departments
of Chemistry and MB&B on a fee-per-use basis.
Notably, this facilitiy houses a 800 MHz Varian Inova NMR spectrometer
equipped with a triple-resonance (HCN) room temperature probe and a 500
MHz Varian Inova NMR spectrometer equipped with a triple-resonance
(HCN) cryogenic probe for significantly enhanced signal-to-noise data
acquisition.
Yale Medical School also houses a state-of-the-art Macromolecular X-Ray
Crystallography Facility in use by multiple researchers from a
variety of Departments. As part of the Yale Cancer Center, our
laboratory has access to a number of Shared Resource Facilities.
Lastly, the W.M. Keck
Foundation Biotechnology Resource Center at Yale offers
comprehensive Genomic and Proteomic services and houses a Biophysics Resource Center
managed by an experienced molecular biophysicist. Instrumentation
available at the resource center includes dynamic light scattering
(DLS), multi-angle laser light scattering coupled to size-exclusion
chromatography (SEC-MALLS), isothermal titration calorimetry (ITC), a
spectrofluorimeter, a fluorescence polarization system, a stopped-flow
spectrofluorimeter, and a Biacore 2000 surface plasmon resonance (SPR)
automated analysis system.
