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Hodsdon Laboratory -- Facilities YSM logo

Our laboratory consists of a 960 square-foot wet bench laboratory fully equipped for recombinant DNA subcloning and mutagenesis; protein expression, refolding and purification; steady-state enzymology and cell culture. We also maintain a number of Linux workstations for NMR spectral processing, analysis and protein computational biology. 

As part of the Magnetic Resonance Research Center (MRRC) at the Yale Medical Center, we maintain and have primary access to a 600 MHz Varian Inova NMR spectrometer equipped with four identical radiofrequency (RF) channels, triple-axis (XYZ) pulsed field gradients, and a triple-resonance (HCN) room temperature probe.

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We also share access to a 500 MHz Bruker Avance NMR spectrometer equipped with three identical radiofrequency (RF) channels, triple-axis (XYZ) pulsed field gradients, and an automation unit consisting of a robotic sample changer and a triple-resonance (HCN) room temperature probe with a robotic tuning interface.

A variety of addtional NMR spectrometers are housed in the Departments of Chemistry and MB&B on a fee-per-use basis. Notably, this facilitiy houses a 800 MHz Varian Inova NMR spectrometer equipped with a triple-resonance (HCN) room temperature probe and a 500 MHz Varian Inova NMR spectrometer equipped with a triple-resonance (HCN) cryogenic probe for significantly enhanced signal-to-noise data acquisition.

Yale Medical School also houses a state-of-the-art Macromolecular X-Ray Crystallography Facility in use by multiple researchers from a variety of Departments. As part of the Yale Cancer Center, our laboratory has access to a number of Shared Resource Facilities. Lastly, the W.M. Keck Foundation Biotechnology Resource Center at Yale offers comprehensive Genomic and Proteomic services and houses a Biophysics Resource Center managed by an experienced molecular biophysicist. Instrumentation available at the resource center includes dynamic light scattering (DLS), multi-angle laser light scattering coupled to size-exclusion chromatography (SEC-MALLS), isothermal titration calorimetry (ITC), a spectrofluorimeter, a fluorescence polarization system, a stopped-flow spectrofluorimeter, and a Biacore 2000 surface plasmon resonance (SPR) automated analysis system.




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