Our laboratory consists of a 960 square-foot wet bench laboratory fully
equipped for recombinant DNA subcloning and mutagenesis; protein
expression, refolding and purification; steady-state enzymology and
cell culture. We also maintain a number of Linux workstations for NMR
spectral processing, analysis and protein computational biology.
As part of the Magnetic Resonance Research Center (MRRC) at the Yale Medical Center, we
maintain and have primary access to a 600 MHz Varian Inova NMR
spectrometer equipped with four identical radiofrequency (RF) channels,
triple-axis (XYZ) pulsed field gradients, and a triple-resonance (HCN)
room temperature probe.
We also share access to a 500 MHz Bruker Avance
NMR spectrometer equipped with three identical radiofrequency (RF)
channels, triple-axis (XYZ)
pulsed field gradients, and an automation unit consisting of a robotic
sample changer and a triple-resonance (HCN) room temperature
probe with a robotic tuning interface.
A variety of addtional NMR spectrometers are housed in the Departments
of Chemistry and MB&B on a fee-per-use basis.
Notably, this facilitiy houses a 800 MHz Varian Inova NMR spectrometer
equipped with a triple-resonance (HCN) room temperature probe and a 500
MHz Varian Inova NMR spectrometer equipped with a triple-resonance
(HCN) cryogenic probe for significantly enhanced signal-to-noise data
acquisition.
Yale Medical School also houses a state-of-the-art Macromolecular X-Ray
Crystallography Facility in use by multiple researchers from a
variety of Departments. As part of the Yale Cancer Center, our
laboratory has access to a number of Shared Resource Facilities.
Lastly, the W.M. Keck
Foundation Biotechnology Resource Center at Yale offers
comprehensive Genomic and Proteomic services and houses a Biophysics Resource Center
managed by an experienced molecular biophysicist. Instrumentation
available at the resource center includes dynamic light scattering
(DLS), multi-angle laser light scattering coupled to size-exclusion
chromatography (SEC-MALLS), isothermal titration calorimetry (ITC), a
spectrofluorimeter, a fluorescence polarization system, a stopped-flow
spectrofluorimeter, and a Biacore 2000 surface plasmon resonance (SPR)
automated analysis system.
